TECHNICAL SPECIFICATIONS:
Wavelength Range | 190-1100nm |
Spectral Bandwidth | 0.5/1/1.5/2/4/5nm Variable |
Optical System | Double Beam, Blazed Holographic Grating (1200 lines/mm) |
Wavelength Accuracy | ±0.5nm |
Wavelength Repeatability | ≤0.2nm |
Wavelength Setting | Auto, Resolution 0.1nm |
Photometric Range | 0~200%T,-4~4A,0~9999C |
Photometric Accuracy | ±0.002 A (0~0.5A), ±0.003A (0.5~1A), ±0.3%T (0~100%T) |
Photometric Repeatability | ≤0.001 A (0~0.5A), ≤0.002A (0.5~1A), ≤0.2%T (0~100%T) |
Stray Light | ≤0.05%T(220/360nm) |
Scan Speed | High, Medium, Low. Max.2000nm/minute |
Baseline Flatness | ±0.001A |
Stability | ±0.001A/h (500nm,0A) |
Noise | ≤0.2%T/3min (250/500nm,0%T); ≤0.3%T/3min (250/500nm,100%T) |
Sample Compartment | 10mm Pathlength Cuvette |
Detector | Silicon Photodiode |
Lamps | Tungsten Lamp & Deuterium Lamp (Pre-aligned) |
Display | Graphic LCD (320*240 Dots) |
Keypad | 30-key Alphanumeric Membrance Keypad |
Output Port | USB Port |
Printer | Mini Serial Printer; PC Printer |
PC Software | PC Scanning Software |
Power Requirements | AC 90-250V, 50/60Hz |
Dimension | 635x515x255mm |
Weight | 26kg |
SALIENT FEATURES:
Double beam ensure low drift, low noise and Low stray light |
Function:
Photometric |
Basic Mode:
To measure the Absorbance and transmittance
Quantitative:
1. Coefficient Method
2. Standard Curve Up to 10 Standard sample may be used to establish a curve. Four methods for fitting a curve through the calibration points :
Linear fit. Linear fit through zero, Square fit and cubic fit.
DNA/Protein Test:
Concentration and DNA purity are quickly and easily calculated: Absorbance rations: 260 nm / 280 nm with optional subtracted absorbance at 320 nm. DNA
concentration = 62.9XA260-36.0XA280 Protein concentration = 1552xA260-
757.3xA 280
Wavelength Scan:
1. The wavelength scan intervals are 0.1,0.2,0.5,1,2,5 nm
2. High, Medium and low scan speed are available. They vary from 100 to 3600 nm/min
3. Wavelength are scanned from high to low so that the instrument waits at high WL. And it minimizes the degradation of UV sensitive samples.
Kinetics:
Abs vs time graphs is displayed on the screen in real time wait time and measurement time up to 12 hours may be entered with time interval of 0.5,1,2,5,10,30 seconds and one min. Post-run manipulation includes re-scalling, curve tracking and selection of the part of the curve required for rate calculation. Rate is calculated using a linear regression algorithm before multiplying be the entered factor.